Two-time women's champion Elina Svitolina has crashed out of the Italian Open, while a knee injury has forced Serena Williams to pull out of the tournament.
Fifth seed Svitolina lost 6-4,1-6, 7-5 against Victoria Azarenka in the second round, while Serena announced her withdrawal just before her match against older sister Venus.
Czech fourth seed Karolína Plisková also advanced to the third round along with Australian eighth seed Ashleigh Barty.
In the men's competition, world no. 5 Alexander Zverev suffered a shock 7-5, 7-5 defeat to Italian wildcard Matteo Berrettini, while Belgian David Goffin rallied from a set down to beat Stan Wawrinka 4-6, 6-0, 6-2.
Ninth seed Marin Cilic, seventh seed Juan Martin Del Potro, Australian Nick Kyrgios and Portugal's Joao Sousa are also through to the second round.
Coventry City ended their 25-year absence from the Premier League with a 1-1 draw at Blackburn Rovers, securing promotion from the Championship with three games to spare on Friday.
Carlos Alcaraz will miss the Madrid Open for the second consecutive year after withdrawing on Friday with a wrist injury, dealing another blow to the world number two's clay court preparations ahead of his French Open title defence.
Novak Djokovic is still working through the injury problems that forced him to miss recent tournaments in Miami and Monte Carlo and said on Friday that he will not feature at next week's Madrid Open, one of the last stops before the French Open.
Mumbai Indians head coach Mahela Jayawardene has defended Jasprit Bumrah after the struggling pace spearhead went a fifth Indian Premier League match without a wicket in Thursday's seven-wicket defeat by Punjab Kings.
Iranian women's football players Fatemeh Pasandideh and Atefeh Ramezanisadeh thanked the Australian government on Friday for offering a "safe haven" and said they wished to resume their sporting careers after rebuilding their lives.