Spain has closed its airspace to US planes involved in attacks on Iran, a step beyond its previous denial of use of jointly-operated military bases, Defence Minister Margarita Robles said on Monday.
"We don't authorise either the use of military bases or the use of airspace for actions related to the war in Iran," she told reporters in Madrid.
Spanish newspaper El Pais had first reported the news on Monday, citing military sources.
The closure of the airspace forces military planes to bypass NATO member Spain en route to their targets in the Middle East, but it does not include emergency situations, El Pais added.
"This decision is part of the decision already made by the Spanish government not to participate in or contribute to a war which was initiated unilaterally and against international law," Economy Minister Carlos Cuerpo said during an interview with radio Cadena Ser when asked if the decision to close Spain's airspace could worsen relations with the United States.
Spanish Prime Minister Pedro Sanchez has been one of the most vocal opponents of the US and Israeli attacks on Iran, describing them as reckless and illegal.
President Donald Trump has threatened to cut trade with Madrid for denying the US use of Spain's bases in the war.
Eight Israeli airstrikes have hit the Abu al-Duhur military airbase in northwestern Syria early on Tuesday, targeting the runway and storage facilities, Syria's state-run Ekhbariya said, citing a military source.
Two students were killed in a shooting at a high school in the southern Philippines on Tuesday, one of them the gunman, officials said, in the second such incident in the country in less than two months.
The Supreme Court of Pakistan has ordered on Tuesday that former Prime Minister Imran Khan be moved to hospital from jail, his Pakistan Tehreek-e-Insaf party said, meeting a longstanding demand of his party and family, who had raised concerns about his health.
A Russian missile attack has killed 10 people and injured a further eight in a village in Ukraine's northeastern Kharkiv region, the regional governor said on Tuesday.
President Donald Trump said on Monday Iran would not make the deal he thinks is needed to end a war with the US and should surrender as talks between the countries remained stalled.