Briton Emma Raducanu was unable to overcome a back injury which forced her to retire from her first round match against Bianca Andreescu at the Italian Open.
Raducanu, who had also struggled with a back injury during her previous tournament in Madrid and a hip injury earlier this year, was trailing 6-2 2-1 when she called it quits.
The battle of the reigning and former US Open champions on clay looked an intriguing prospect but Raducanu struggled from the outset and served poorly, handing Andreescu a number of break points.
Raducanu was clutching her back when she took a medical timeout after going 5-2 down but returned to the court minutes later where the Canadian held to love to take the opening set.
The 19-year-old Raducanu held serve at the start of the second set but looked uncomfortable yet again and this time she took the decision to retire when she was down 2-1 in the second.
Raducanu, who played on clay professionally for the first time last month, said she did not want to make a hasty decision to skip the French Open and focus on the grasscourt swing and her home Grand Slam at Wimbledon.
Meanwhile, Tunisian Ons Jabeur, who became the first African player to win a WTA 1000 title in Madrid over the weekend, survived a second set wobble to dispatch Romania's Sorana Cirstea 6-0 7-6(1).
Jabeur looked set for a comfortable victory at 5-2 up in the second with 45 minutes played.
However, Cirstea fought back valiantly as Jabeur lost her focus and made a slew of errors, forcing a tiebreak where the Tunisian eventually prevailed to win her seventh consecutive match for the first time in her career.